General Information of This Target
Target ID
BTDT10166
Target Name
Nicotinic acetylcholine receptor (nAChR) chimeric alpha-7
Target Bioclass
Receptor
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N.A.
Toxin Information Related to This Target
                           Toxin Name Activity Data Type Activity Data Reference
 Toxin Info    Erabutoxin a Dissociation constant
0.07 nM
[1- 13]
 Toxin Info    Alpha-bungarotoxin Dissociation constant
0.95 nM
[8- 34]
 Toxin Info    Alpha-bungarotoxin, isoform A31 Dissociation constant
0.95 nM
[35], [36]
 Toxin Info    Alpha-elapitoxin-Nno2a Dissociation constant
12 nM
[8- 40]
 Toxin Info    Erabutoxin b Dissociation constant
22 μM
[2- 48]
References
Ref 1 Cloning and sequence analysis of the cDNA encoding a snake neurotoxin precursor. Biochimie. 1985 Feb;67(2):185-9. doi: 10.1016/s0300-9084(85)80046-8.
Ref 2 Molecular evolution and diversification of snake toxin genes, revealed by analysis of intron sequences. Gene. 2003 Aug 14;313:111-8. doi: 10.1016/s0378-1119(03)00637-1.
Ref 3 The amino acid sequences of erabutoxins, neurotoxic proteins of sea-snake (Laticauda semifasciata) venom. Biochem J. 1971 May;122(4):453-61. doi: 10.1042/bj1220453.
Ref 4 Correction of partial amino acid sequence of erabutoxins. Biochem J. 1977 Oct 1;167(1):289-91. doi: 10.1042/bj1670289.
Ref 5 The disulphide bonds of erabutoxin a, a neurotoxic protein of a sea-snake (Laticauda semifasciata) venom. Biochem J. 1971 May;122(4):463-7. doi: 10.1042/bj1220463.
Ref 6 Genetic engineering of snake toxins. Role of invariant residues in the structural and functional properties of a curaremimetic toxin, as probed by site-directed mutagenesis. J Biol Chem. 1993 Jan 15;268(2):909-16.
Ref 7 Genetic engineering of snake toxins. The functional site of Erabutoxin a, as delineated by site-directed mutagenesis, includes variant residues. J Biol Chem. 1995 Apr 21;270(16):9362-9. doi: 10.1074/jbc.270.16.9362.
Ref 8 Only snake curaremimetic toxins with a fifth disulfide bond have high affinity for the neuronal alpha7 nicotinic receptor. J Biol Chem. 1997 Sep 26;272(39):24279-86. doi: 10.1074/jbc.272.39.24279.
Ref 9 Effects of alpha-erabutoxin, alpha-bungarotoxin, alpha-cobratoxin and fasciculin on the nicotine-evoked release of dopamine in the rat striatum in vivo. Neurochem Int. 1998 Oct;33(4):307-12. doi: 10.1016/s0197-0186(98)00033-3.
Ref 10 Three-dimensional structure of neurotoxin a from venom of the Philippines sea snake. Proc Natl Acad Sci U S A. 1977 Mar;74(3):971-4. doi: 10.1073/pnas.74.3.971.
Ref 11 The crystal structure of erabutoxin a at 2.0-A resolution. J Biol Chem. 1989 Jun 5;264(16):9239-42. doi: 10.2210/pdb5ebx/pdb.
Ref 12 Structure of dimeric and monomeric erabutoxin a refined at 1.5 A resolution. Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):964-74. doi: 10.1107/s0907444998005125.
Ref 13 High resolution x-ray analysis of two mutants of a curaremimetic snake toxin. Eur J Biochem. 2000 Mar;267(5):1323-9. doi: 10.1046/j.1432-1327.2000.01099.x.
Ref 14 Genetic characterization of the mRNAs encoding alpha-bungarotoxin: isoforms and RNA editing in Bungarus multicinctus gland cells. Nucleic Acids Res. 1998 Dec 15;26(24):5624-9. doi: 10.1093/nar/26.24.5624.
Ref 15 Genetic organization of alpha-bungarotoxins from Bungarus multicinctus (Taiwan banded krait): evidence showing that the production of alpha-bungarotoxin isotoxins is not derived from edited mRNAs. Nucleic Acids Res. 1999 Oct 15;27(20):3970-5. doi: 10.1093/nar/27.20.3970.
Ref 16 Purification, properties and amino acid sequence of -bungarotoxin from the venom of Bungarus multicinctus. Hoppe Seylers Z Physiol Chem. 1972 Feb;353(2):243-62. doi: 10.1515/bchm2.1972.353.1.243.
Ref 17 Primary structure of alpha-bungarotoxin. Six amino acid residues differ from the previously reported sequence. FEBS Lett. 1987 Sep 28;222(1):79-82. doi: 10.1016/0014-5793(87)80195-3.
Ref 18 Isolation of antigenically reactive peptide fragments and localization of antigenic regions of alpha-bungarotoxin. Biochem Biophys Res Commun. 1988 Sep 15;155(2):870-6. doi: 10.1016/s0006-291x(88)80576-x.
Ref 19 cDNA sequence analysis and expression of alpha-bungarotoxin from Taiwan banded krait (Bungarus multicinctus). Biochem Biophys Res Commun. 1995 Nov 22;216(3):1088-94. doi: 10.1006/bbrc.1995.2732.
Ref 20 Structural studies of alpha-bungarotoxin. 3. Corrections in the primary sequence and X-ray structure and characterization of an isotoxic alpha-bungarotoxin. Biochemistry. 1988 Apr 19;27(8):2775-81. doi: 10.1021/bi00408a018.
Ref 21 Neutralizing monoclonal antibody specific for alpha-bungarotoxin: preparation and characterization of the antibody, and localization of antigenic region of alpha-bungarotoxin. FEBS Lett. 1989 Aug 28;254(1-2):106-10. doi: 10.1016/0014-5793(89)81018-x.
Ref 22 The cholinergic antagonist alpha-bungarotoxin also binds and blocks a subset of GABA receptors. Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5149-54. doi: 10.1073/pnas.0600847103. Epub 2006 Mar 20.
Ref 23 Neurotoxins from snake venoms and -conotoxin ImI inhibit functionally active ionotropic -aminobutyric acid (GABA) receptors. J Biol Chem. 2015 Sep 11;290(37):22747-58. doi: 10.1074/jbc.M115.648824. Epub 2015 Jul 28.
Ref 24 Snake neurotoxin -bungarotoxin is an antagonist at native GABA(A) receptors. Neuropharmacology. 2015 Jun;93:28-40. doi: 10.1016/j.neuropharm.2015.01.001. Epub 2015 Jan 26.
Ref 25 The crystal structure of alpha-bungarotoxin at 2.5 A resolution: relation to solution structure and binding to acetylcholine receptor. Protein Eng. 1986 Oct-Nov;1(1):37-46. doi: 10.1093/protein/1.1.37.
Ref 26 Structural studies of alpha-bungarotoxin. 1. Sequence-specific 1H NMR resonance assignments. Biochemistry. 1988 Apr 19;27(8):2763-71. doi: 10.1021/bi00408a016.
Ref 27 Structural studies of alpha-bungarotoxin. 2. 1H NMR assignments via an improved relayed coherence transfer nuclear overhauser enhancement experiment. Biochemistry. 1988 Apr 19;27(8):2772-5. doi: 10.1021/bi00408a017.
Ref 28 NMR solution structure of an alpha-bungarotoxin/nicotinic receptor peptide complex. Biochemistry. 1993 Nov 23;32(46):12290-8. doi: 10.1021/bi00097a004.
Ref 29 Three-dimensional solution structure of the complex of alpha-bungarotoxin with a library-derived peptide. Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6059-64. doi: 10.1073/pnas.94.12.6059.
Ref 30 The solution structure of the complex formed between alpha-bungarotoxin and an 18-mer cognate peptide derived from the alpha 1 subunit of the nicotinic acetylcholine receptor from Torpedo californica. J Biol Chem. 2001 Jun 22;276(25):22930-40. doi: 10.1074/jbc.M102300200. Epub 2001 Apr 18.
Ref 31 A beta -hairpin structure in a 13-mer peptide that binds alpha -bungarotoxin with high affinity and neutralizes its toxicity. Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6629-34. doi: 10.1073/pnas.111164298. Epub 2001 May 29.
Ref 32 NMR structure of alpha-bungarotoxin free and bound to a mimotope of the nicotinic acetylcholine receptor. Biochemistry. 2002 Feb 5;41(5):1457-63. doi: 10.1021/bi011012f.
Ref 33 NMR structural analysis of alpha-bungarotoxin and its complex with the principal alpha-neurotoxin-binding sequence on the alpha 7 subunit of a neuronal nicotinic acetylcholine receptor. J Biol Chem. 2002 Apr 5;277(14):12406-17. doi: 10.1074/jbc.M110320200. Epub 2002 Jan 14.
Ref 34 The mechanism for acetylcholine receptor inhibition by alpha-neurotoxins and species-specific resistance to alpha-bungarotoxin revealed by NMR. Neuron. 2002 Jul 18;35(2):319-32. doi: 10.1016/s0896-6273(02)00773-0.
Ref 35 Identification of alpha-bungarotoxin (A31) as the major postsynaptic neurotoxin, and complete nucleotide identity of a genomic DNA of Bungarus candidus from Java with exons of the Bungarus multicinctus alpha-bungarotoxin (A31) gene. Toxicon. 2003 Sep 15;42(4):381-90. doi: 10.1016/s0041-0101(03)00168-5.
Ref 36 Novel long-chain neurotoxins from Bungarus candidus distinguish the two binding sites in muscle-type nicotinic acetylcholine receptors. Biochem J. 2019 Apr 26;476(8):1285-1302. doi: 10.1042/BCJ20180909.
Ref 37 Amino acid sequence of neurotoxin I from Naja naja oxiana venom. FEBS Lett. 1974 Sep 1;45(1):118-21. doi: 10.1016/0014-5793(74)80825-2.
Ref 38 Crystallization and preliminary x-ray diffraction study of neurotoxin-I from Naja naja oxiana venom. FEBS Lett. 1990 Aug 20;269(1):255-7. doi: 10.1016/0014-5793(90)81167-m.
Ref 39 Three-dimensional structure of neurotoxin-1 from Naja naja oxiana venom at 1.9 A resolution. FEBS Lett. 1993 Apr 5;320(2):111-7. doi: 10.1016/0014-5793(93)80073-4.
Ref 40 Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy. Eur J Biochem. 2004 Dec;271(23-24):4950-7. doi: 10.1111/j.1432-1033.2004.04465.x.
Ref 41 Sequence analysis of a cDNA encoding a erabutoxin b from the sea-snake Laticauda semifasciata. Nucleic Acids Res. 1989 Dec 25;17(24):10490. doi: 10.1093/nar/17.24.10490.
Ref 42 Protein sequencing by computer graphics. Biochim Biophys Acta. 1977 Apr 25;491(2):605-8. doi: 10.1016/0005-2795(77)90309-9.
Ref 43 The crystal structure of a post-synaptic neurotoxin from sea snake at A resolution. FEBS Lett. 1976 Sep 15;68(1):1-4. doi: 10.1016/0014-5793(76)80390-0.
Ref 44 Structure and function of snake venom curarimimetic neurotoxins. Mol Pharmacol. 1978 Jul;14(4):710-6.
Ref 45 Molecular conformation of erabutoxin b; atomic coordinates at 2.5 A resolution. Biochem Biophys Res Commun. 1979 Jun 13;88(3):950-9. doi: 10.1016/0006-291x(79)91500-6.
Ref 46 Erabutoxin b. Structure/function relationships following initial protein refinement at 0.140-nm resolution. Eur J Biochem. 1986 Dec 15;161(3):579-87. doi: 10.1111/j.1432-1033.1986.tb10481.x.
Ref 47 Structure determination of a dimeric form of erabutoxin-b, crystallized from a thiocyanate solution. Acta Crystallogr B. 1992 Aug 1;48 ( Pt 4):520-31. doi: 10.1107/s010876819200096x.
Ref 48 Tertiary structure of erabutoxin b in aqueous solution as elucidated by two-dimensional nuclear magnetic resonance. J Mol Biol. 1994 Jul 8;240(2):155-66. doi: 10.1006/jmbi.1994.1429.
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