BioTD (Biotoxin Database) is an innovatively developed database dedicated to provide comprehensive toxins information from multiple perspective. BioTD includes 14,607 data records and provides 8,975 toxins from 5,213 references and patents. These toxins come from more than 900 species, such as snakes, spiders, scorpions, snails, and others, making it a comprehensive toxins database. Moreover, 8,185 activity data manually curated from literature articles. The activity data of BioTD can be divided into five categories: Activity, Safety, Kinetics, Hemolysis, and other Physiological indicators. Various activity information of toxins against different species and targets is systematically collected. Overall, BioTD is of significant interest to biologists across various disciplines, owing to its diverse applications in fields such as medicine, neuroscience, pharmacology, drug discovery, and agriculture which aim to facilitate toxin research.